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Chromatin states of developmentally-regulated genes revealed by DNA and histone methylation patterns in zebrafish embryos

Reverse transcription polymerase chain reaction (RT-PCR) Reverse transcription polymerase chain reaction was performed as previously described on 23 NK ligands [15]

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Journal of Ethnopharmacology , 118 (3), 373377

Cells were blocked with 1% BSA in PBS for 30 min at RT, then incubated with primary antibodies (anti-Flag (1:200) and anti-Sororin (1:100)) at 37 C for 1 h