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To dissociate biotinylated proteins from streptavidin, boil the beads in 0.1% SDS or SDS-PAGE buffer for 3 mins or incubate them in 8 M guanidinium hydrochloride, pH 1.5

This fact is disputed by some authors who believe that a significant proportion of people with normal or high levels of vitamin B12 actually have a deficiency [14, 19, 25]