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HC estimates are used as levels of concern protective to a broad array of aquatic species, as they inform of potential impacts and are used to derive PNECs because they are a community-level threshold making full use of the available data on the toxicity of a chemical to standard and non-standard toxicity test species and so relate concentration to ecological impacts (i.e., the proportion of species at risk)

The reference range for B12 can vary from lab to lab but it is usually between 200 700 pmol/l or pg/ml if you are in the U.S.A

However, vitamin B12 can sometimes be difficult to absorb from food or pills, or we simply do not have enough vitamin B12 in our diet

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