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Mass spectrometrical analysis of recombinant human growth hormone (Genotropin) reveals amino acid substitutions in 2% of the expressed protein

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10.7524/AJE.1673-5897.20200304001 41 DongS.LiuX.WangY

With the increasing F1 peptide concentrations, from 0.8 to 3.2 M, the V max value decreased from 0.833 mol/mg/min to 0.645 mol/mg/min, K i value increased from 4.0 M to 5.82 M, and [I]/K i value increased from 0.2 to 0.55

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