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For cytokine/chemokine measurements, 17-Plex, duplex and single-plex analyte detection panels were run on the samples using Human Luminex Multiplex Immunoassay kits (R&D Systems)

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The high uric acid concentration in non-injected chicks could result from increased liver metabolic processes owing to imbalanced amino acids 30

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The enzyme activity was assessed by determining the rate constant (k) of a first-order reaction mathematical equation