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Several studies employing single-cell RNA sequencing, mass cytometry, and fluorescence cytometry revealed phenotypic alterations in microglia across various models, although spatial information was limitedly obtained and the pathological mechanisms remained unclear at that time

MTX clearance is PH dependent, and this lifesaving fact is used to treat its increased plasma concentrations via urine alkalinization [74]

In neuroinflammation, SFRP1 upregulates hypoxia-inducible factor (HIF)-dependent inflammatory pathways, promoting microglial activation (Rueda-Carrasco et al., 2021)