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The His6-tagged protein sample was eluted with an elution buffer (20 mM Tris-HCl (pH 8.0), 300 mM NaCl, 10% (v/v) glycerol, 0.25% (w/v) DM and 300 mM imidazole) and further purified by size-exclusion chromatography (Superdex 200 from GE Healthcare) in a crystallization buffer (20 mM Tris-HCl (pH 7.5), 100 mM NaCl and 0.6% (w/v) OG (Anatrace))

Rapid progress is being made in this field, and long-term efficacy and safety studies are underway

Orlando (FL) Konstan MW, Doring G, Lands LC, et al

After RNA extraction with the help of DNaseI, DNA digestion was performed

Cisplatin-resistant cells exhibit increased fatty acid uptake and favor beta-oxidation to survive under cisplatin-induced oxidative stress [29]