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The initiation of lipid peroxidation necessitates the abstraction of diallyl hydrogen atoms positioned between the two carbon-carbon double bonds from polyunsaturated PUFA-PL on phospholipids, resulting in the formation of carbon-centered free radicals (PL-) that subsequently react with molecular oxygen to generate lipid peroxy radicals (PLOO-)

N., Shilton, A., & Guieysse, B

PMCID: PMC4967041

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Indo-1 signals were immediately recorded after adding PMA (100 nM), A23187 (1.25 M), LPS (10 g/mL), or anti-CD16 crosslinking for 5 min