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In this context, PON1 activity may be more appropriately interpreted as a complementary indicator that reflects one dimension of the oxidative and metabolic milieu, rather than as a standalone diagnostic tool

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Optimization of test conditions In order to obtain the best detection results, the parameters affecting the glutathione determination as well as the IR signal enhancement, including the mixing ratio of glutathione solution and silver nanoparticles as well as the reaction time, the drying temperature and drying time of the coated infrared window slice of the samples to be measured, were optimized in this study