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2016;24(7):575-584 Kaufmann CN

Essential tremor: epidemiology, diagnosis and treatment

The reaction mixture (3 mL) contained 50 mM TrisHCl buffer (pH 8.2), 0.1 mM EDTA, 13 mM L-methionine, 75 M NBT, 2 M riboflavin, and an appropriate volume of enzyme extract (typically 2050 L)