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Purification of the crude by semi-preparative HPLC (eluent A: 0.1% TFA in water, eluent B: 0.1% TFA in CH 3 CN, 20 mL min 1 , 010% eluent B in 5 min then 1035% in 30 min, C18 column, detection at 215 nm, 30 C) furnished SetCys-K1[150176]-MPA peptide 13 as a white solid after lyophilization (34.7 mg, 17%)

In insulin-resistant THP-1 macrophages, lauric acid enhanced mitochondrial biogenesis and glucose uptake at concentrations of 5-50 M (24 h), thereby increasing the expression of PGC-1, TFAM, GLUT1, and GLUT-3, thereby attenuating insulin resistance and promoting metabolic health ( By contrast, myristic acid is associated with increased body weight, adipose tissue inflammation, and insulin resistance, contributing to the metabolic disease progression

Nucleic Acids Res 35:W429W432 Kang WH, Park YH, Park HM (2010) The LAMMER kinase homolog, Lkh1, regulates Tup transcriptional repressors through phosphorylation in Schizosaccharomyces pombe

By contrast, the presence of the inhibitor forces the Tyr296 side chain to a closed conformation, hindering the NADPH attachment, and leading to enzyme inhibition

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