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doi: 10.1021/acs.jafc.9b04442

RNAi-expressing bacteria were allowed to grow overnight at 20 C

The crispr-process-nf Nextflow workflow is available from GitHub ( For statistical analysis, we used the crispr-mageck-nf Nextflow workflow, also available from GitHub ( To calculate gene-level enrichment, the sorted populations (ENL/BRD4s high or ENL/BRD4s low ) were compared to the ENL/BRD4s mid populations in MAGeCK (version 0.5.9) 114 using median-normalized read counts