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The cells were cultured in six-well plates, and then were collected 48 h post-drug treatment, washed with PBS, and lysed for 2 min with 0.25% trypsin (Gibco, Grand Island, USA)

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While these mechanisms are well-supported by in vitro and animal studies, human epidemiological data show variability due to confounders like exposure dose and genetic background, highlighting the need for larger, prospective cohorts to resolve conflicting findings

Studies have shown several benefits to these types of diets, including a decreased risk of obesity, hypertension, diabetes, cancer, and even a decreased risk of severe COVID-19, however, these diets are known to lack in specific nutrients, bringing with them added health risks due to nutrient deficiencies

Ros and diseases: role in metabolism and energy supply