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Moreover, it was shown experimentally that Gch1 (gene encoding the rate-limiting enzyme of BH4 biosynthesis) is a direct target of Nrf-2 in skin cells submitted to radiation (Nrf-2 has a binding site in the proximal promoter of the Gch1 gene), and the resultant increased intracellular BH4 levels promoted cytoprotection by neutralizing ROS [108]

A timer was started once the experimenter released the mouse to allow it to hang freely

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