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This conservation is not surprising as oxidative stress is an inevitable byproduct of aerobic respiration, and GSH is an important antioxidant that mitigates the effects of reactive oxygen species (ROS), such as hydrogen peroxide

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Chromatographic separation was achieved on a Platinum EPS C18 100 A 3 u (length: 53 mm, internal diameter: 7 mm, maximum pressure: 5000 PSIG) analytical column (Alltech Associates, Inc., Deerfield, Ill.) at ambient temperature with a flow rate of the mobile phase maintained at 1 ml/min