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Effect of NNMT inhibitor on adipocyte differentiation quantitated with oil red O staining 3T3-L1 cells were cultured in 60 mm diameter dishes (8.410 4 cells/dish) and treated with NNMT inhibitor dissolved in culture media with/without adipogenic factors (1 mM IBMX, 1 M dexamethasone, 10 g/ml of insulin) during each of the scheduled media changes during the differentiation process (described above)

Compounded versions may be more accessible, though they are not identical to the brand-name product and quality can vary between compounding pharmacies

For broader context on aging biology and metabolic decline research, the NIH National Institute on Aging provides authoritative resources on the metabolic changes and epigenetic mechanisms that underlie age-associated obesity and sarcopenia

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