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[DOI] [PMC free article] [PubMed] [Google Scholar] 33.Ding S., OBanion C.P., Welfare J.G., Lawrence D.S

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DNA degradation through this harsh treatment involving extreme temperatures and pH is the major disadvantage of this sequencing technique, but there are also another setbacks such as low specificity, low DNA sequence diversity through the short fragments and difficult haplotyping (because short reads have a smaller probability of containing single-nucleotide polymorphisms (SNP) than longer strands) [17, 42]

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