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Storage Lyophilizate (dry powder before reconstitution) 3 years at 20 C (freezer) 2 years at 2 to 8 C (refrigerator) Only short-term (up to 7 days) at room temperature, NAD+ is less stable than peptides Protect from light and moisture (extremely hygroscopic) After reconstitution (NAD+ in solution) Only 7 to 14 days at 2 to 8 C , NAD+ has a shorter shelf life in solution than peptides A NAD+ solution is especially sensitive to light, heat, and pH changes For long-term storage after reconstitution: freeze in aliquots at 20 C , use within 3 months Practical storage rules Let the vial warm to room temperature (15 to 20 min) before opening

Hydrogen Bond To achieve and confirm the M pro glutathione complex stability, and the irreversible attachment to it, intermolecular hydrogen bonds analysis are mandatory to check, thus, hydrogen bonds were evaluated for their number and distribution, and were shown in Fig

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Han, Y., Lan, N., Pang, C