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Measurement of lipid peroxidation, glutathione peroxidase activity, total urinary protein and insulin To assess lipid peroxidation (4-Hydroxynonenal (4-HNE)), 50 mg of LV sample (endocardium-anterior) was suspended in a lysing tube (845-CS-1140250, Innuscreen GmbH, Berlin, Germany) with 750 L RIPA buffer (89,901, ThermoFisher, Waltham, USA) and homogenized using a homogenizer (Speedmill Plus, Analytik Jena GmbH, Jena, Germany)

In rare cases, clinicians recommend liver biopsy to evaluate damage

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In parallel, we address the emerging field of chrononutrition, emphasizing that the timing of food intake and consumption of melatonin-rich or polyphenol-containing foods can enhance circadian rhythmicity, improve sleep quality, and reduce age-related metabolic and oxidative stress