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Pellets were resuspended in 3 mL of assay buffer (250 mM KCl, 2 mM MgCl 2 , 40 mM HEPES-KOH pH 7.9, 5 mM -mercaptoethanol, 10% (v/v) glycerol, 10 mM imidazole), and 1.25 mL of this resuspension was then used in the pull-down

10.3389/fpls.2016.01325 Front

Other types of estrogen include estrone, which becomes the dominant form after menopause, and estriol, which is produced during pregnancy

These enhancements have been achieved by manipulating the nutritional content of poultry diets to strike a balance (Lobo et al ., 2010)

This combination of storage and turnover introduces a temporal delay between inadequate intake or absorption and the onset of deficiency