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Along with the counteracted increase of pro-inflammatory cytokines such as interleukin-6, tumor necrosis factor-, looking at muscle metabolism relevant to cancer cachexia, BPC 157 counteracted muscle wasting, significantly corrected deranged muscle proliferation as well as myogenesis, the changes in the expression of transcription factor (FoxO3a), phospho-AKT (p-AKT), phospho mammalian target of rapamycin (p-mTOR), and phospho glycogen synthase kinase 3 (p-GSK-3)

RNA extraction and RTqPCR Total RNA from cells was isolated via TRIzol (Takara, Otsu, Japan) and subsequently purified with chloroform/isopropanol/ethanol, and cDNA was synthesized via the PrimeScript RT reagent Kit (Takara) according to the manufacturers instructions and used as the template for qRTPCR

IEEE Trans

1997;25:S557

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