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Purification procedure For phage purification, 1 ml of filtered lysates was mixed with 1 ml of LB medium and incubated with 5 ml of glutathione Sepharose (GE Healthcare) or Ni-NTA agarose (QiaGen) overnight at 4C

Protein expression, purification and verification The plasmid encoding His-tagged NCOA4 (pET28a-His-NCOA4 383522 ) was transformed into Escherichia coli BL21 (DE3) competent cells