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The discrepancy may be attributed to the distinct metabolic characteristics of cells and the variations in the induction approaches used in different studies 18,23

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doi: 10.3390/ijms22179541 191 de Almeida ChuffaLGLupiLACucieloMSSilveiraHSReiterRJSeivaFRF

& Fujita, D

If deproteinization of plasma prior to storage is necessary for prevention of spontaneous oxidation, then there would have been no difference in levels of GSH between Preparation Condition 1 and Preparation Condition 2, and we would have found no difference in level of total GSH and differences in levels of free GSH, GSSG, and their relative ratio between Preparation Condition 3 and Preparation Condition 4