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Notably, the secretory phase is subdivided into three transcriptionally defined stages based on clustering analysis of epithelial single-cell transcriptomes, to ensure consistency with the original publicly available scRNA-seq dataset

2.8.2 Human hepatocarcinoma cell line For cell viability in the human hepatocarcinoma cell line (HepG2), the 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay was performed as previously described ( 2