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Blood samples were collected 60 minutes after removal from room temperature, centrifuged to obtain serum, and analyzed using an electrochemiluminescence immunoassay

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3.2.3 Sex hormones abnormalities Many research has discovered sex differences in the prevalence of both GD and depression, with a reported male-to-female ratio of 1:4-6 in GD and a ratio of roughly 1:2 in major depressive disorder (122, 128, 129)