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For cyto- and genotoxicity testing, isolated hepatocytes (2 10 5 per well) were seeded in 0.5 ml of Dulbeccos Modified Eagle Medium (DMEM) with low glucose (Thermo Fisher Scientific, Waltham MA, USA) supplemented with 10% fetal calf serum (FCS, Pan-Biotech, Aidenbach, Germany) and 1% Penicillin/Streptomycin solution (Thermo Fisher Scientific, Waltham MA, USA) per well on rat tail collagen-coated 24-well plates at 37 C in a humidified atmosphere with 5% CO 2