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For each well of a 12-well plate, 2575 pmol siRNA was transfected using 5 L Lipofectamine RNAiMAX

Furthermore, Nrf2 promoter hypermethylation is observed in both COPD patients and CSE-treated HBE cells, resulting in NRF2 downregulation, inhibited GPX4 expression and ferroptosis occurrence[21]

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