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Not much is know about the upstream signaling cascade

doi: 10.1016/j.clnu.2009.01.015 [DOI] [PubMed] [Google Scholar] 92.Mahmoodianfard S, Vafa M, Golgiri F, Khoshniat M, Gohari M, Solati Z, et al

In all, 1 mL of 10 mM Tris [pH 10.5] was added to each well to solubilize SRB dye, and the OD at 510 nm was measured in a microplate reader (Tecan Infinite M200Pro)