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Since supernatant isolated from the WT had a higher pH, contained low levels of GSH, and restored gsh2 mutant growth, we reasoned that L-DOPA medium conditioned with WT cells could be used to recover gsh2 melanin production

To confirm that the SAT1 flipper disruption cassettes had correctly replaced either the GDH2 or GDH3 gene after double crossover integrative transformation, the 5 and 3 junctions of disrupted alleles were PCR amplified