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Sample preparation for erythrocyte lysate analysis commenced by centrifugation of the 2-mL portion at 4C, 2500 g for 5 min

Sekhar KR, Cyr S, Baregamian N

Jaya-Ram A, Shu-Chien AC, Kuah MK

Our work demonstrates that HIF-2, via an increase in cellular iron, is an important source of ROS, which coupled to posttranslational protein modification by DMF or lipid-ROS induction by ferroptosis-inducing agents, leads to cell death (Figure 8F)

In G6PD deficient RBCs, it has been shown that after treatment with diamide, a specific thiol oxidant, or divicine band 3 is rapidly oxidized and becomes irreversibly phosphorylated