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The cells were then cultured in a 12-well dish containing a poly-L-lysine-coated coverslip in glucose-free DMEM for another 1 h in a humidified chamber with 5% CO 2 at 37 C, for cells to attach to the coverslips

Pharmacol Res 46:499503 Bae EH, Lee KS, Lee J, Ma SK, Kim NH, Choi KC, Frokiaer J, Nielsen S, Kim SY, Kim SZ, Kim SH, Kim SW (2008) Effects of alpha-lipoic acid on ischemia-reperfusion-induced renal dysfunction in rats