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Therefore, a common aspect of this protocol is the sample pre-treatment with NEM, which should be done as soon as possible after its collection

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The disulfide can be recycled to a dithiol by Trx-reductase (TrxR), thereby oxidizing one molecule of NADPH 17

Mutations in mTORC1 containing RAPTOR F1296E/M1297E alone, mTOR K471D/R472D/K474D alone or the combination of both reduced mTORC1 kinase activity (Fig