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The extent of any model overfitting during refinement was measured by refining the final model against one of the half-maps and by comparing the resulting map versus model FSC curves with the two half-maps and the full model

RRID: AB_314449) in 0.1 ml incubation buffer for 30 min at 25C

commercial extract during the aging of middle age wistar rats: differential effects on the hippocampus and striatum

Using the approach of Habig et al

demonstrated that Erastin treatment in fibroblasts and fibrosarcoma cells robustly induced the conversion of LC3I to LC3II and the formation of GFP-LC3 puncta, which are definitive hallmarks of autophagy activation (49)