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ACS Chemical Neuroscience Pontrello CG, McWhirt JM, Glabe CG, Brewer GJ (2022) Age-related oxidative redox and metabolic changes precede Intraneuronal amyloid-beta Accumulation and Plaque Deposition in a transgenic Alzheimers Disease Mouse Model

For the AMPylation activity of the selected members of the S-HxxxE family proteins, 1 g His 6 -LnaB or homologous proteins and 0.5 g actin were incubated in a 20 L reaction system containing 50 mM Tris-HCl (pH 7.5), 5 mM MgCl 2 and 5 Ci ATP-- 32 P for 2 h at 37 C