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Cells were washed twice in ice-cold PBS and lysed for 15 min on ice with RIPA buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1% Triton X-100, 0.5% sodium deoxycholate and 0.1% SDS) supplemented with Benzonase (25 U per ml) and 1 Halt protease inhibitor cocktail

Pacing Clin Electrophysiol 2020;43:828837

doi: 10.1021/acs.analchem.7b04527 145 HsuBETabariesSJohnsonRMAndrzejewskiSSenecalJLehuedeCet al

CrossRef Resveratrol regulates skeletal muscle fibers switching through the AdipoR1-AMPK-PGC-1 pathway Qinyang Jiang, Xiaofang Cheng, Yueyue Cui, Qin Xia, Xueyu Yan, Mingyuan Zhang, Ganqiu Lan, Jiaqi Liu, Tizhong Shan, Yanna Huang Food & Function.2019

Additionally, granulosa cells in women with endometriosis exhibit apoptosis, mitochondrial dysfunction, disrupted steroid hormone production, and increased inflammatory markers, further compromising follicle health ( These changes contribute to significant oocyte quality defects