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For pseudo-TIRFM, the calculation of the penetration depth was performed by measuring the position at which the beam was exiting from the center of the objective and subsequently correlated to the penetration depth versus the displacement of the beam from the center using the exponential decay depth equation described previously 73 , incorporated into the Nikon software

In vitro platinum cell uptake Cells were seeded at 1.5 10 5 cells/well in six-well plates in 1.5 mL and after 24 h recovery treated with 500 L of compounds at the indicated concentrations in triplicates

m o ) Representative whole dorsal striatal images of SST immunofluorescence from Trkb Penk-WT control ( m ), Trkb Penk-KO mutant injected at 3 M with Gsto2 -shRNA ( n ) or scrambled-shRNA in the striatum ( o ) and analysed at 15 W

Additional file 5: Supplemental Table S2 (download DOCX

Purified rhGPx3 mutant was incubated with factor Xa protease (1 g/50 g fusion protein in a total reaction volume of 50 l) for 48 h at 4C