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PI cannot penetrate intact cell membranes and thus only stains cells whose membrane integrity is compromised, such as apoptotic or necrotic cells

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Lipolysis in the primary adipocytes of Insr P1195L/ + mice is suppressed under ND but increased under HFD P1195L/ + To exclude the influence of the sympathetic nervous system and hyperinsulinemia in Insr P1195L/ + mice, we measured glycerol release in primary adipocytes in vitro

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