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After serum limitation-induction, positive FOXO4 and p53-pS15 immunofluorescence staining was stronger in the nucleus of keloid fibroblasts at 48 h, but in the nucleus of normal skin fibroblasts at 72 h

[DOI] [PMC free article] [PubMed] [Google Scholar] 172.Martnez-Mora A., Soto-Urquieta M.G., Robles E.F., Ziga-Trujillo I., Campos-Cervantes A., Perez-Vazquez V., Ramrez-Emiliano J

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Cells were lysed with NIB-250 buffer (15 mM Tris-HCl (pH 7.5), 60 mM KCl, 15 mM NaCl, 5 mM MgCl 2 , 1 mM CaCl 2 , 250 mM sucrose, 1 mM DTT, 10 mM sodium butyrate, protease inhibitors) with 0.1% NP-40 for 5 minutes on ice

who demonstrated that kidneys undergoing a short period of NMP had increased expression of heat shock protein 70 and IL-6 together with improved metabolic function and less tubular injury compared with static cold-stored kidneys (182)