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The mouse anti-GAPDH antibody (ab8245, 6C5, GR3275542-1), mouse anti-GGT antibody (ab55138, 1F9, GR1017756-3), rabbit anti-Calreticulin antibody (ab2907, GR3426057-2), rat anti-CD8 antibody (ab25478, 53-6.7, GR3181210-4), goat anti-rabbit IgG H&L (HRP) (ab6721), goat anti-rabbit IgG H&L (Alexa Fluor 488) (ab150077), and goat anti-rabbit IgG H&L (Alexa Fluor 555) (ab150078) were purchased from Abcam (Shanghai, China)

Logan S, Pharaoh GA, Marlin MC, Masser DR, Matsuzaki S, Wronowski B, Yeganeh A, Parks EE, Premkumar P, Farley JA, Owen DB, Humphries KM, Kinter M, Freeman WM, Szweda LI, Van Remmen H, Sonntag WE (2018) Insulin-like growth factor receptor signaling regulates working memory, mitochondrial metabolism, and amyloid- uptake in astrocytes

Non-targeted lipidomicsinstrumental condition and data analysis The HPLC conditions were identical to the previous study 97

At the indicated times post-infection, coverslips were washed once with PBS and fixed in 4% paraformaldehyde (Ted Pella Inc., 18505, diluted in 1 x PBS) for 10 min at room temperature

for 30 seconds