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The supernatant was collected and stored on ice, when used immediately, or frozen at 80 C for future use

Under Cd treatment, the TBARS content in Tiannong 9 was 24.7, 6.4, 3.37 and 6.28% higher than that of Fenghe 6 at 1st, 2nd, 3rd and 4th day after treatment, respectively

If necessary, the eluted protein can be concentrated using ultrafiltration or dialysis against an appropriate buffer to remove excess glutathione and prepare the protein for downstream applications