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doi:10.1161/CIRCRESAHA.109.198366, PMID: 19910579 Starkov AA, Fiskum G, Chinopoulos C, Lorenzo BJ, Browne SE, Patel MS, Beal MF (2004) Mitochondrial alpha-ketoglutarate dehydrogenase complex generates reactive oxygen species

To remove cell debris, the plates were centrifuged for 10 min at 3500 RPM and 4 C, before the supernatants were collected

In the early stage of ALI, PAMPs such as LPS promote the polarization of lung macrophages into the M1 phenotype through the TLR4/NF-B signaling pathway (119)