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Mice were initially group housed (three/cage) and allowed to acclimate to the colony environment maintained at a constant temperature (21-23 C.) and humidity (40-50%) on a 12-hour light-dark cycle (lights on 0600-1800 h)

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These cytokines are markers of chronic low-grade inflammation, the kind associated with accelerated biological aging, cardiovascular disease, neurodegeneration, and metabolic syndrome

The study provides a valuable insight into the governance conditions required for CBCA implementation and highlights the best practices from leading cities