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Unlike growth hormone, IGF-1 influences how cells respond to available energy rather than how fat breaks down

This was performed by treating cells with or without an mTORC1 activator (insulin, 15 minutes treatment), and by additionally treating with or without an mTORC1 inhibitor (rapamycin) for three hours in insulin-deprived cells (repeated three times)

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J Gene Med 7:14591467 Backliwal G, Hildinger M, Hasija V, Wurm FM (2008) High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI

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