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Description

The formation of HBP was monitored by measuring the absorbance at 475 nm 26

Traditional detection employs 2,4-dinitrophenylhydrazine (DNPH) derivatization, forming stable adducts quantifiable spectrophotometrically or via anti-DNPH antibody Western blotting (Madian and Regnier, 2010)

[DOI] [PMC free article] [PubMed] [Google Scholar] 38.Li T., Chen Y., Gua C., Li X

doi: 10.1016/j.freeradbiomed.2006.06.024

This finding suggests an alternative reaction mechanism characterized by three distinct redox states (GPX4-SeH, GPX4-SeOH, and GPX4-Se-OO - ) involving the catalytically active selenocysteine