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The hGAN-G332R mice had increased IF protein levels and displayed giant swollen axons with densely packed IFs

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Western blot analysis To assess protein expression, cells were pelleted at 10,000 g for 2 min and resuspended in B-PER (Thermo Scientific) lysis buffer (supplemented with 100 g/mL lysozyme, 20 g/mL DNase I, and 20 g/mL RNase A) according to the equation: volume of lysis buffer (L) = volume of culture (mL) x OD 600 of culture x 100