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5mCH methylatation differences occur around neuronal transcription factor binding sites and around transcription start sites, suggesting that it is sufficient to cause transcriptional repression in neurons, as effectively as, but independently of, 5-mCG [38]

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3), it was insufficient to maintain mTORC1 pathway and suppress the ISR during prolonged cystine limitation (Fig

37th European Peptide Symposium 14th International Peptide Symposium , Florence, Italy, 25 - 29 August 2024

Immunoblotting Cell lysates were extracted in 1 RIPA lysis and extraction buffer (Sigma-Aldrich Chemie GmbH R0278) containing 2 Protease and Phosphatase Inhibitor Cocktail (Thermo Scientific 78444) for 20 min on ice