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Samples were then washed and exposed to PI (1 mg/ml) for 15 min at 37C, washed and mounted using Vectashield prior to examination by Leica TCS SP5 CLSM microscopy

However, these studies were limited by the toxicity of H 2 O 2 and the poor tissue permeability of BxxP, a membrane-impermeant variant of BP required to prevent labeling of intracellular ER and Golgi proteins by surface-targeted HRP 3

GCMS analysis of SCFAs Targeted metabolomics was performed with GCMS to quantify the concentrations of SCFAs in cecal content