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10.3389/fmicb.2012.00030 Front

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Samples were separated on SDSPAGE gels, transferred to PVDF membranes and blocked in 2% fatty acid-free BSA and 5% skim milk powder in PBS containing 0.2% Tween-20 (PBST) for lipoate detection, or 5% skim milk powder in PBST for glutaryl-lysine detection

For Alzheimer disease, the current FDA-approved treatments include donepezil, rivastigmine, galantamine, memantine, and more recently lecanemab and donanemab (anti-amyloid antibodies with modest clinical effects)