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2.4 Sample preparation For LCQTOF-MS/MS analysis, 1 mg of powder or homogenized tablet was dissolved or extracted using 5 mL of a 1:1:1 (v/v/v) mixture of water/methanol/acetonitrile, assisted by ultrasonication for 10 min, and afterwards filtered by a Whatman 0.2 m pore size polytetrafluoroethylene (PTFE) filter medium (GE Healthcare, Chicago, IL, United States)

Due to the effect of Alpha lipoic acid reducing blood sugar levels, it is required that you eat well before the IV as we check your blood before and again after administration of the IV and a snack is also beneficial to maintain blood sugar levels

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However, it should be noted that one study failed to find beneficial effects of vitamin D supplementation in patients with ASD, although sample size in this trial is relatively small 42

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