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The free energy profiles of the afatinib reaction in both EGFR and solution demonstrate a classic Michael addition in which the NA step is rate-limiting and a stable intermediate is formed before reprotonation to the thiol adduct state

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Oxidative stress is a condition of disturbed redox homeostasis either by excessive production of reactive oxygen species (ROS) or reactive nitrogen species (RNS), or by suppressed antioxidant mechanisms

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